As part of the project „Oxford Nanopore Technology: Enzyme Optimization and Genomic Data Analysis for Commercial Applications”, the first DNA sequencing experiment using Oxford Nanopore technology was conducted at the Department of Complex Systems. The experiment was divided into two parts - the experimental one carried out at the Department of Biotechnology and Bioinformatics of the Faculty of Chemistry and the bioinformatics carried out at the Department of Complex Systems of the Faculty of Electrical and Computer Engineering. The efficient sequencing as well as correct analysis of the obtained data was supervised by Jan Gawor from the Laboratory of DNA Sequencing and Oligonucleotide Synthesis at the Institute of Biochemistry and Biophysics of the Polish Academy of Sciences. The following persons were also involved in the experiment: PhD Marta Sochacka-Piętal, PhD Michał Pietal, MSc Małgorzata Semik, MSc Michał Wroński, PhD Dominik Strzałka, MSc Magdalena Totoń, Ehg. Michał Ćmil and MSc Anna Czmil. The necessary organizational and technical assistance was also provided by PhD DSc Eng. Mirosław Tyrka, prof. PRz. The starting material in the experimental part was DNA from thermotolerant and thermophilic strains of Bacillus genus. During the bioinformatic part of the experiment, the project participiants acquainted with a number of bioinformatic tools (including Bandage, Guppy, NanoPlot, Fly, Canu) used in de novo assembly of bacterial genomes. As a result of the experiment, de novo complete genomic sequences of the analyzed Bacillus sp. Strains were obtained.
More on www page: https://zsz.prz.edu.pl/dzialalnosc-naukowa/projekty/technologia-oxford-nanopore/aktualnosci
